How to preserve bacterial strains

Bacteria are a necessary condition for growing some fungus vegetables. Only good bacteria can grow high-quality fungus vegetables. After purchasing or making bacteria, its preservation is particularly important, otherwise it will lose its activity and sometimes mutate, which is not good for fungus cultivation. So how should bacteria be preserved? There are many different ways to preserve bacteria, and I will write them out and tell you about them later.

Methods for preservation of bacterial strains

1. Bacteria can be stored on a slant
When preserving bacterial strains, you can use a slant for preservation. The slant preservation here means inoculating the bacterial strain on the slant culture medium. After it grows completely, move it to the refrigerator for refrigeration. After a period of time, use new slant culture medium to cultivate it, and then refrigerate it again. Repeating this process can achieve good preservation results.
2. The strains can be preserved with petroleum wax seals
After the bacteria are cultured on the culture medium, they can be sealed with liquid paraffin, which can also achieve a good preservation effect. After wrapping the bacteria with paraffin, they also need to be placed in a place with a temperature below five degrees. Too high a temperature will reduce the preservation quality. In addition, the paraffin used to preserve the bacteria must be sterilized.
3. The strains can be frozen and vacuum preserved
Frozen vacuum preservation is also an important method for people to preserve bacterial strains, also known as freeze-drying. This method is to protect the prepared bacterial strains with a protective agent, and then put them into a low-temperature environment to freeze them quickly and extract all the air inside. In this way, the bacterial strains can be in a vacuum and low-temperature environment, which can put the microorganisms in a dormant state. When needed, they can be taken out and placed in a warm environment to restore the activity of the bacterial strains.

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