How to grow Haworthia stripedii? Breeding methods and techniques of Haworthia stripedii

It is popular to plant succulents now. The planting method of succulents is simple, and the later management and maintenance are not cumbersome, so many people like to plant succulents. Today I will introduce you to Haworthia striata. So, as a beginner, what steps should you pay attention to when planting Haworthia striata? Next, we will introduce to you how to grow Haworthia striata.

How to grow Haworthia striata

How to reproduce Haworthia striata

Division and cuttings are commonly used for propagation. Division can be done all year round. When repotting in April and May, the young plants around the mother plant are peeled off and directly potted. Cuttings are best done between May and June. Cut the fleshy leaves with a little semi-lignified part and insert them into a clean river sand substrate. They will take root in about 20-25 days. It is best to transplant them into culture soil when the roots are 2-3 cm long. Division can be done all year round in an indoor environment, but it is most suitable in April and May each year. Cut the young plants next to the mother plant directly, dry them slightly, and plant them in another pot.

Propagation by division

Division time: It is best to do it in early spring (February or March) after the soil thaws.
Division method: Take the mother plant out of the pot, shake off excess soil, separate the tangled roots as much as possible, and use a sharp knife to split it into two or more plants. Each plant must have a considerable root system, and its leaves should be properly trimmed to facilitate survival.

Disinfection of pots

Soak the divided plants in 1500 times dilution of chlorothalonil for five minutes, take them out and let them dry, then you can pot them. You can also use chlorothalonil to irrigate the roots immediately after potting.

Management after division

After the division, irrigate the roots or water thoroughly once. Because its root system is severely damaged and its water absorption capacity is extremely weak, it takes about 3 to 4 weeks to recover and sprout new roots. Therefore, watering should be restrained within 3 to 4 weeks after division to prevent root rot, but the transpiration of its leaves is not affected. In order to maintain the water balance of the leaves, the leaves need to be sprayed 1 to 3 times a day (more spraying when the temperature is high, less spraying or no spraying when the temperature is low). Do not apply fertilizer during this period. After division, you should also pay attention to the strong sunlight. It is best to place it in a shade shed for maintenance.

Tissue culture rapid propagation

1. Materials: Young shoots from the roots of Haworthia striata stems (Plant cells are omnipotent, that is, any plant cell has the ability to form the plant body. In other words, any highly differentiated cell has the ability to revert to the original undifferentiated cell and can differentiate into other forms of highly differentiated cells. This "omnipotence" is the theoretical basis of plant tissue culture. Based on this characteristic of plant cells, any highly differentiated cell of the plant body can be cultured arbitrarily, and the young shoots are the highly differentiated parts, so they are chosen).

2. Culture conditions: MS is used as the basic culture medium. (Ingredients: KNO3: 1650 NH4NO3: 1900 MgSO4.7H2O: 370 KH2PO4: 170 CaCl2.2H2O: 440 MnSO4.4H2O: 22.3 ZnSO4.7H2O: 8.6 H3BO3: 6.2 NaMoO4.2H2O: 0.25 CuSO4.5H2O: 0.025 Na2.EDTA: 37.3 FeSO4.7H2O: 27.8 Inositol: 100 Nicotinic acid: 0.5 Glycine: 2 Thiamine hydrochloride: 0.1 Pyridoxine hydrochloride: 0.5 KI: 0.83 CoCl2.6H2O: 0.025 Sucrose)

Cut the axillary buds with 4-5 young leaves from the stems of the mother plants of potted Haworthia fasciata. Rinse with running water for 5 minutes, soak in 0.2% Chlorhexidine for 30 minutes, and rinse with running water for 10 minutes (this method is more water-saving and has a very low pollution rate). Place the material on a clean bench and disinfect it with 0.1% HgCl2 for 8 minutes, rinse with sterile water 10 times, and dry it with sterile filter paper. Cut the bud part with a scalpel and divide the cut young leaves into small pieces of 0.5 cm. Inoculate it into the culture medium for cultivation.

3. Bud culture: The buds of Haworthia striata swell and grow rapidly on the culture medium. After about 20 days, the elongated buds are cut into 3-4 parts and inoculated into another culture medium. After another week, a group of young buds (about 5-10) sprout at the base of each bud.

4. Callus and bud induction: After the young leaf segments were cultured in the culture medium for about 2 weeks, a layer of colorless and transparent callus was produced on the wound surface. After 20 days of culture, the callus did not proliferate but 2-3 small buds grew from it. After the buds were cut off and transferred to another culture medium, a group of seedlings was formed.

5. Root induction and transformation: The young leaf segments were placed in D and cultured for one month. Many white bulbous roots were formed at the wound and continued to increase. These roots were transferred to culture medium for about a week. The roots turned green and callus tissue grew. After being transferred to another culture medium for two weeks, bulbous embryos were produced, and some embryos began to grow young leaves.

6. Subculture and rapid propagation: The obtained in vitro propagation system of the plant can achieve geometric proliferation through repeated separation and micro-cutting. This process is called rapid micropropagation or rapid propagation. Rapid plant propagation is usually carried out after the in vitro propagation system is established. Sometimes the callus tissue can also be rapidly proliferated. At this time, liquid suspension culture is usually used. The small seedling clusters or spherical embryos obtained above can be roughly divided and inoculated into another culture medium for large-scale proliferation, doubling every two weeks on average. Using a bud as an exosome, more than 20 bottles of subculture plants (about 30 plants per bottle) have been obtained after two months.

7. Rooting of strong seedlings: Inoculate the large seedlings in culture medium E, increase the light to 10h/d, and take root after one week. After another week of cultivation, they can be transplanted. (When the tissue culture technology is used to build the overall form of a certain plant, that is, to obtain seedlings with stems, roots, and leaves, they must undergo certain seedling hardening treatments to gradually transition from a sterile environment to a bacterial environment, and from an artificial culture environment to a natural environment. It is customary to adopt the "transition treatment method", that is, gradually change the environment, such as using sterilized soil, moisture retention, and heat preservation for bottle seedlings, and at the same time, certain seedling hardening treatments must be done, such as increasing light and supplementing nutrient solution. After a series of treatments, a "clone seedling" cultured from tissue culture will survive successfully.)

8. Transplantation: Open the bottle before taking out the seedlings and harden them for two days. Wash the culture medium of the rooted seedlings and transfer them to the sterile matrix of vermiculite + perlite, maintain humidity, and the survival rate is higher than 90%.